YRNAs are small non-coding RNAs, around 100 nucleotides long, that play essential roles in DNA replication, RNA stability, and overall cellular balance. These molecules are increasingly recognized for their importance in health and disease. In cancer, YRNAs and their smaller fragments, called YsRNAs, often show altered levels, influencing cell growth, division, and stress responses. Because their behavior differs between healthy and diseased tissues, YRNAs hold great promise as novel biomarkers, opening new avenues for research and discovery.
High-quality RNA extraction is essential to explore these molecules effectively and has become a foundational step in many molecular biology, transcriptomics, and diagnostics workflows. On the contrary, Poor RNA quality or low yield can compromise downstream applications like qRT-PCR, RNA-seq, microarrays, or detection of low-abundance transcripts.
This is where Cytiva’s RNAspin Kits—especially the RNAspin Mini Kit and RNAspin 96 Kit—come into play. Whether you’re working with a few samples or running high-throughput studies, Cytiva’s Amersham™ RNAspin kits help you get reliable and reproducible RNA preparations.
We Offer Cytiva’s Complete Solutions for YRNA Research
From small-scale experiments to high-throughput screening, Cytiva’s RNAspin kits deliver consistent, high-quality RNA ready for applications like qRT-PCR and microarray analysis. When paired with best practices and technical support, these kits help unlock reliable RNA outputs and stronger downstream analyses. Whether your focus is YRNA function, biomarker discovery, or disease research, these kits streamline workflows and ensure reliable results.
RNAspin Mini Kit – Precision for small samples
The RNAspin Mini Kit is designed for rapid extraction of high-quality total RNA from a wide range of sample types, without needing plate handling or automation.
- Extract up to 100 µg of high-quality RNA from as few as 10 HeLa cells or small tissue samples.
- On-column DNase I treatment ensures pure RNA free of genomic DNA.
- Prefilters reduce lysate viscosity, improving yield and preventing column clogging.
- Simple and convenient format, suitable for all levels of expertise.
RNAspin 96 Kit – High-throughput efficiency
The RNAspin 96 Kit ensures fast and efficient total RNA extraction from cultured cells, tissue, bacteria or yeast, reducing hands-on time significantly.
- Process up to 96 samples in parallel in under 70 minutes.
- Reproducible RNA isolation with typical RIN > 8.0, suitable for sensitive downstream applications.
- Compatible with manual, vacuum, centrifugation, or automated workflows.
- DNase I treatment included, with prefilters to reduce lysate viscosity and maintain purity.
Both kits have DNase treatment to prevent gDNA contamination, an important factor for assays like qRT-PCR or RNA-seq where background DNA can interfere.
How The Science Support Helps You Implement the RNAspin Kits
Implementing the RNAspin kits with maximum efficiency for your lab often requires more than just purchasing reagents. Here’s how we assist:
- Protocol optimization: Adapting the lysis method or sample input depending on tissue type, cell numbers, or presence of tough bacterial strains.
- Automation or batching support: For labs processing many samples, setting up vacuum manifolds or liquid-handling platforms to run RNAspin 96 in plate format.
- Quality control: Ensuring downstream QC (spectrophotometry, gel/Northern, or Bioanalyzer) to check RIN, absence of DNA, etc.
- Training & technical service: Hands-on training for lab staff, troubleshooting yield or purity issues, advice on RNA storage and handling. Find out more about our support services.
If your lab is considering upgrading its RNA extraction workflow, whether to get more samples done, improve consistency, or just get cleaner input, we can help you pick, implement, and optimize the right RNAspin solution for your needs.
Source:
Guglas, K., Kołodziejczak, I., Kolenda, T., Kopczyńska, M., Teresiak, A., Sobocińska, J., Bliźniak, R., & Lamperska, K. (2020). YRNAs and YRNA-Derived Fragments as New Players in Cancer Research and Their Potential Role in Diagnostics. International Journal of Molecular Sciences, 21(16), 5682. https://doi.org/10.3390/ijms21165682